![]() ![]() Embryonic stem cells come from a blastocyst that is 4–5 days old. The blastocyst contains stem cells and will later implant in the womb. Embryonic stem cellsįrom the very earliest stage of pregnancy, after the sperm fertilizes the egg, an embryo forms.Īround 3–5 days after a sperm fertilizes an egg, the embryo takes the form of a blastocyst or ball of cells. However, some evidence now suggests that they can differentiate to become other cell types, as well. In the past, scientists believed adult stem cells could only differentiate based on their tissue of origin. This division and regeneration are how a skin wound heals, or how an organ such as the liver, for example, can repair itself after damage. This means they can generate various cell types from the originating organ or even regenerate the original organ, entirely. They can stay non-dividing and non-specific for years until the body summons them to repair or grow new tissue.Īdult stem cells can divide or self-renew indefinitely. However, stem cells can be difficult to find. Scientists have found stem cells in tissues, including: ![]() Stem cells are present inside different types of tissue. In some parts of the body, such as the gut and bone marrow, stem cells regularly divide to produce new body tissues for maintenance and repair. They remain in this state until the body needs them for a specific purpose, say, as skin or muscle cells.ĭay-to-day living means the body is constantly renewing its tissues. The cells are in a non-specific state, but they are more specialized than embryonic stem cells. ![]() The body can use these stem cells whenever it needs them.Īlso called tissue-specific or somatic stem cells, adult stem cells exist throughout the body from the time an embryo develops. This extended protocol uses a complete T cell progenitor maturation medium made with reagents included in the StemSpan™ T Cell Generation Kit and must be combined with ImmunoCult™ CD3/CD28/CD2 T Cell Activator and IL-15.Share on Pinterest Stem cells can turn into any type of cell before they become differentiated.Ī person’s body contains stem cells throughout their life. StemSpan™ Lymphoid Progenitor Expansion Supplement (10X) and StemSpan™ T Cell Progenitor Maturation Supplement (10X) are intended for use in combination with StemSpan™ SFEM II medium, and on plates coated with StemSpan™ Lymphoid Differentiation Coating Material (100X).Īn optional protocol is available to further mature DP cells to CD8 SP T cells. It also supports the maturation of DP cells to CD8 single-positive (SP) T cells with additional stimuli. Additionally, StemSpan™ T Cell Progenitor Maturation Supplement (10X) enables further maturation of pro-T cells to DP cells. StemSpan™ Lymphoid Progenitor Expansion Supplement (10X) contains a combination of recombinant human cytokines and other additives formulated to selectively promote the expansion and differentiation of CD34 + cells isolated from human CB samples to CD7 +CD5 + progenitor T (pro-T) cells. This medium kit is serum- and feeder-free, eliminating variation introduced by serum and stromal cell lines.įor your convenience, all kit components are also available for purchase individually. In step two, pro-T cells generated during those first 14 days are cultured for another 4 weeks in medium containing maturation supplement to promote their maturation into DP cells. In step one, CD34 + CB cells are cultured for 14 days in medium containing expansion supplement to promote their proliferation and differentiation into CD7 +CD5 + pro-T cells. Use this two-step protocol to reproducibly expand thousands of CD4 +CD8 + double-positive (DP) T cells in cultures initiated with CD34 + human cord blood (CB) cells. Tissue and Cell Culture Dissociation Reagents.Work at STEMCELL View Current Opportunities > ![]()
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